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Functional and Cellular Analysis Platform



FLOW CYTOMETRY LABORATORY

Technical Staff:
Marta Soler
E-mail: MSolerC@santpau.cat / citometria@santpau.cat

Functions:


Conventional cytometry:

In homogenous (cell line) or heterogeneous (biological fluid) samples:
  • Detection of the presence of proteins and determine of cell populations (cell markers).
  • Studies of signalling cascades (cell cycle, cell proliferation, apoptosis, intracellular calcium determination).
  • Quantification of transfection efficiency.
  • Phenotype changes. Relative and absolute presence.
  • Production of mediators.
  • Detection of minority populations.
Cell separator:

In sub-populations of separated cells:
  • Microarrays.
  • Western blot.
  • Cell cultures.
  • RT PCR.
Equipment:
  • FACSCalibur (BD) conventional flow cytometry.
  • MACSQuant (Milteny Biotech) conventional flow cytometry.
  • FACSAria (BD) flow cytometry cell sorter.
Grants:
  • HSCSP Research Institute. "Flow cytometry cell sorter: analysis system and high throughput cell sorting". IF06/3678-1. Joint financing: FEDER.


CELL CULTURE LABORATORY

Technical Staff:
Elena Serrano
E-mail: eserrano@santpau.cat

Functions:
To make adequate facilities and equipment available to researchers working with cell cultures in a BSL2 laboratory.

Equipment:
  • 4 CO² incubators.
  • 2 hypoxia incubators (2-21% O²).
  • 2 IIA biosafety cabinets (BSL2).
  • 2 hypoxia cabinets (BSL1).
  • Culture baths.
  • Olympus inverted microscope with high-resolution colour camera.
  • Refrigerated centrifuge.
  • Fridge 4ºC and freezer -20ºC.
  • Thermo-desinfector and autoclave.

Platform coordinator:
Elena Serrano
E-mail: eserrano@santpau.cat
Tel: +34 93 553 72 25


PRICE LIST 2016

FORM CIT FLUX

FORM BSL2 CULTURES


 

 



 

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